Device

Part:BBa_K5078009:Design

Designed by: Callie Ross   Group: iGEM24_UVU-Utah-2   (2024-10-01)


pL2 -aadA-nosZ(P.stu)-Ptc amiRNA-Psr1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3752
    Illegal PstI site found at 5477
    Illegal PstI site found at 6963
    Illegal PstI site found at 7056
    Illegal PstI site found at 8636
    Illegal PstI site found at 9123
    Illegal PstI site found at 9332
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4514
    Illegal NheI site found at 6936
    Illegal NheI site found at 10148
    Illegal NheI site found at 10904
    Illegal PstI site found at 3752
    Illegal PstI site found at 5477
    Illegal PstI site found at 6963
    Illegal PstI site found at 7056
    Illegal PstI site found at 8636
    Illegal PstI site found at 9123
    Illegal PstI site found at 9332
    Illegal NotI site found at 3356
    Illegal NotI site found at 11367
    Illegal NotI site found at 11457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4731
    Illegal BglII site found at 11778
    Illegal BamHI site found at 3678
    Illegal BamHI site found at 12155
    Illegal XhoI site found at 3245
    Illegal XhoI site found at 4799
    Illegal XhoI site found at 6783
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3752
    Illegal PstI site found at 5477
    Illegal PstI site found at 6963
    Illegal PstI site found at 7056
    Illegal PstI site found at 8636
    Illegal PstI site found at 9123
    Illegal PstI site found at 9332
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3752
    Illegal PstI site found at 5477
    Illegal PstI site found at 6963
    Illegal PstI site found at 7056
    Illegal PstI site found at 8636
    Illegal PstI site found at 9123
    Illegal PstI site found at 9332
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 4286
    Illegal NgoMIV site found at 9356
    Illegal NgoMIV site found at 9724
    Illegal NgoMIV site found at 9757
    Illegal AgeI site found at 8655
    Illegal AgeI site found at 9742
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

osdifhoa uhg ksbdv kdhodj a.lfh awefo;vksd


Source

olia for dhheuyryt a f

References